Figure 8.

Roots of Wild-Type and atg4a4b-1 Mutants Treated with Concanamycin A under Nitrogen-Starved Conditions.

(A) to (C) Wild-type roots.

(D) to (F) atg4a4b-1 mutant roots.

(G) and (H) Complemented atg4a4b-1 mutant (atg4a4b-1;tATG4a) roots.

Roots of 1-week-old wild-type ([A] to [C]), atg4a4b-1 ([D] to [F]), and atg4a4b-1 transformed with the ATG4a gene ([G] and [H]) seedlings grown on MS medium were incubated in MS-N liquid medium containing concanamycin A for 12 h and then observed by conventional transmission light microscopy. Bars = 20 μm for (A), (B), and (E) to (H) and 10 μm for (C) and (D).