Table 1.

Summary of Missense Substitutions in the aux1 Allelic Series and Their Properties

Mutation Name	Location/Domaina	WT AAb	Locus (AA No.)c	aux1 AAd	Signaturee	Conservationf	Penetranceg	PHCDh
aux1-120	Tm2	Ser	57	Phe	NDi	100%	Total	155
aux1-112	Tm4	Gly	178	Asp	Potential N-myristoylation site	100% identical	Total	94
aux1-111	Tm5	Ser	215	Phe	ND	100%	Total	155
aux1-104	Tm6	Gly	238	Glu	ND	80%	Total	98
aux1-106	Tm6	Gly	247	Glu	ND	80%	Total	98
aux1-113	Tm6	Ala	250	Thr	ND	80%	Partial	58
aux1-114	Tm6/Tm7 loop	Met	259	Ile	ND	80%	Partial	10
aux1-116	Tm6/Tm7 loop	Pro	262	Ser	ND	80%	Partial	74
aux1-2	Tm7	Ala	272	Val	ND	100%	Partial	64
aux1-117	Tm7/Tm8 loop	Gly	292	Glu	Highly conserved motif in AAAP genes	80%	Total	98
aux1-102	Tm7/Tm8 loop	Met	305	Arg	ND	100%	Total	91
aux1-103	Tm8	Thr	324	Met	ND	80%	Total	81
aux1-118	Tm10	Ser	386	Asn	ND	80%	Total	46
aux1-105	Tm11	Gly	439	Arg	ND	80%	Total	125
aux1-7	C-term tail	Gly	459	Asp	ND	80%	Total	94

^aTm, transmembrane domain.

^bWT AA, wild-type amino acid residue.

^cLocus AA, number of amino acid residue in the AUX1 coding sequence.

^daux1 AA, amino acid substitution in the aux1 allele.

^eSignature, AAAP (amino acid-auxin permease) signature.

^fConservation, conservation over AUX1 and four paralogues (see Fig. 5).

^gPenetrance, severity of genetic defect in the aux1 allele.

^hPHCD, physicochemical distance.

ⁱND, no data.