Figure 2.

Accumulation of Plastid Proteins.

Loading 100 corresponds to 8 μg of membrane or soluble proteins of the wild type. The quantity of apo1 thylakoid membranes (100%) was adjusted to wild-type levels of the ATP synthase. For quantification, dilution series of the wild type were used. Three-week-old wild-type and apo1 leaves were used for analysis.

(A) Immunoblot analysis of thylakoid membrane and soluble plastid proteins. Designations of proteins and of the corresponding complexes are labeled at left and right, respectively.

(B) Immunoblot analysis of the outer PSI and PSII antenna proteins in apo1 and wild type. Lhc, Light-harvesting complex.

(C) Immunoblot analysis of Fe-S cluster–containing plastid proteins. The plastid-encoded thylakoid membrane and the nuclear-encoded soluble protein complexes NDH and FTR, respectively, contain [4Fe-4S] clusters. Ferredoxin (FD) binds [2Fe-2S] clusters.