Chiral Analysis of Linalool and Nerolidol Produced by the Recombinant FaNES1 Protein and Ripe Fruit of the Cultivated Strawberry.
(A) and (D) References of R and S linalool enantiomers and of nerolidol enantiomers; 1 and 2, (3R) and (3S) (Z)-nerolidol (elution order not known); 3, (3R)(E)-nerolidol; 4, (3S)(E)-nerolidol.
(B) and (E) Enzyme activity assays with the recombinant FaNES1 enzyme and either GPP or FPP as substrate.
(C) and (F) Linalool and nerolidol produced by ripe, cultivated strawberry fruit.