Figure 9.

BTI1 Protein Levels Transiently Increase after Agrobacterium Infection of Arabidopsis Suspension Cells.

Plant cells were infected with various Agrobacterium strains (or were mock-inoculated), proteins were extracted at various times, and the proteins were subjected to protein gel blot analysis using anti-BTI1 antibody. The amount of BTI1 protein in each sample was normalized to the amount of actin, as determined by protein gel blot analysis using antiactin antibody. The ratio of BTI1 protein:actin protein was normalized to 1 at 0 h. The green line indicates BTI1 protein levels of uninfected Arabidopsis suspension cell cultures. The red line indicates BTI1 protein levels of Arabidopsis suspension cells infected with Agrobacterium At849 (GV3101 containing pBISN1; this strain can transfer both T-DNA and virulence proteins). The purple line shows BTI1 protein levels of Arabidopsis suspension cells infected with Agrobacterium GV3101 (nononcogenic Agrobacterium strain containing a disarmed pTiC58 plasmid; this strain can transfer virulence proteins but not T-DNA). The blue line indicates BTI1 protein levels of Arabidopsis suspension cells infected with Agrobacterium At793 (pBISN1 in Agrobacterium A136 lacking a Ti-plasmid; this strain can transfer neither virulence proteins nor T-DNA). Data shown in the figure are average values of three independent experiments. Error bars = se.