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. 2017 Jan 9;127(2):564–568. doi: 10.1172/JCI90520

Figure 1. Sol-Mer is increased in advanced human and murine atherosclerotic lesions.

Figure 1

(A) Carotid endarterectomy (CEA) specimens were analyzed for sol-Mer by immunoblot, and necrotic area was measured from H&E-stained images. The correlation coefficient (R) and P value were based on Pearson’s product-moment correlation analysis. (B) CEA specimens from asymptomatic and symptomatic patients were assessed by immunoblot for sol-Mer (n = 5 per group). β-Actin and MAC2 were used as loading controls for total protein and macrophages, respectively. (C) Paraffin-embedded CEA sections were stained with anti-MerTK antibody, imaged by confocal microscopy (scale bar: 30 μm), and quantified by MerTK mean fluorescence intensity (MFI) relative to the asymptomatic cohort (n = 6–8 for each group). *P < 0.05, by 2-tailed Student’s t test. (D) Age-matched Ldlr–/– mice were fed the Western-type diet (WD) for 0, 8, or 16 weeks and sacrificed at 24 weeks of age. The aortic arch, Brachiocephalic Artery (BCA), and descending aorta up to the renal bifurcation were removed en bloc and immunoblotted for sol-Mer (n = 3 per group).