Skip to main content
. 2017 Jan 17;127(2):593–607. doi: 10.1172/JCI87588

Figure 1. Murine Gpr182 expression profile during development and adulthood.

Figure 1

(A) Targeting vector of the Gpr182tm2a(KOMP)Wtsi/+ lacZ reporter mouse model (29, 62, 63). The GPR182 protein–coding region is shaded in pink. mGPR182, murine GPR182. (B) Whole-mount X-gal staining of E8 Gpr182lacZ/lacZ embryo. DA, dorsal aorta; VV, vitelline vein. (C) Optical projection tomography of whole-mount X-gal–stained WT and Gpr182lacZ/lacZ E13.5 embryos. X-Gal staining in Gpr182lacZ/lacZ E13.5 heart (D) and E17.5 lung, kidney, liver, yolk sac, and stomach, pancreas, and duodenum (E). Representative Gpr182 lacZ expression in adult heart (F), lung (G), kidney (H), liver (I), glandular stomach (J), and colon (K) stained with X-gal and/or β-gal (green). Sections were costained with DAPI (purple) and either the endothelial marker CD31 (F) or the podocyte marker podoplanin (PDPN) (H). X-Gal–stained sections were counterstained with eosin (F–H) or Neutral Red (D and I–K). Scale bars: 200 μm (B and D), 1 mm (C, E, and K), and 100 μm (F–J).