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. 2016 Dec 19;127(2):457–471. doi: 10.1172/JCI85145

Figure 4. Silencing or overexpression of CLEC14A regulates the phosphoactivation of VEGFR-3 and VEGFR-2 and their downstream signaling upon stimulation with VEGF-C or VEGF-A in HDBECs.

Figure 4

(A) Decreased phosphoactivation and expression of VEGFR-3 and increased phosphoactivation and expression of VEGFR-2 following increased phosphoactivation of ERK in HDBECs after knockdown of CLEC14A using siRNA (si) (50 nM) and treatment with 50 ng/ml VEGF-A for 5 minutes. (B) Decreased phosphoactivation and expression of VEGFR-3 following decreased phosphoactivation of ERK and increased phosphoactivation and expression of VEGFR-2 in HDBECs after knockdown of CLEC14A using siRNA (50 nM) and treatment with 100 ng/ml VEGF-C for 15 minutes. (C) Elevated phosphoactivation and expression of VEGFR-3 and reduced phosphoactivation and expression of VEGFR-2 following decreased phosphoactivation of ERK in HDBECs after overexpression of GFP-tagged CLEC14A, together with VEGF-A (50 ng/ml) for 5 minutes in HDBECs (GFP-Mock and GFP-CLEC14A-FL). (D) Enhanced phosphoactivation and expression of VEGFR-3 following increased phosphoactivation of ERK and decreased phosphoactivation and expression of VEGFR-2 after overexpression of GFP-tagged CLEC14A, together with VEGF-C (100 ng/ml) treatment for 15 minutes in HDBECs (GFP-Mock and GFP-CLEC14A-FL). All experiments were repeated in at least 4 different sets. M, mock transfected; CL, transfected with CLEC14A.