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. 2016 Dec 19;127(2):472–486. doi: 10.1172/JCI85052

Figure 2. Fluorescent neomycin preferentially enters and kills LL HCs.

Figure 2

(A) A neuromast in a sedated 5 days post fertilization (dpf) zebrafish imaged during chronic exposure to 50 μM Neo-TR. Neo-TR was added to the media at time 0. Neo-TR first enters the kinocilia and stereocilia (6 minutes) and subsequently the HC body in both puncta (yellow arrows) and diffuse pools (9 minutes 30 seconds). Note that non-HCs in the fish do not take up Neo-TR. HCs with high concentrations of Neo-TR show blebbing at the apical region and kinocilia (asterisks: 21 minutes 30 seconds; 28 minutes), and separation of the apical region from the HC body (bracket: 21 minutes 30 seconds). (See also Supplemental Video 3.) Scale bar: 10 μm. (B) Neo-TR time series in a brn3c-GFP transgenic zebrafish, treated as in A. The appearance of Neo-TR on the stereocilia (plus symbol) and kinocilia (asterisk) occurs nearly simultaneously and prior to detectable Neo-TR throughout the HC body (yellow arrow). Scale bar: 10 μm. (See also Supplemental Video 3.)