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. 2017 Jan 17;127(2):635–650. doi: 10.1172/JCI89486

Figure 7. Targeting BCL-W induces apoptosis in human BL.

Figure 7

(A–D) The indicated human BL cell lines were infected with a Dox-inducible lentivirus encoding either a BCL-W shRNA (shBW) or nontargeting (shNT) control shRNA. Following the addition of Dox or DMSO vehicle (Veh) control, Western blotting for cleaved caspase 3 (A), MTS assays (B, in quadruplicate), and staining for subG1 DNA content (C, in triplicate) and annexin V positivity (D, in triplicate) were performed. (E and F) Daudi cells were doubly infected with the Dox-inducible lentivirus expressing either a BCL-W shRNA (shBW) or nontargeting (shNT) control shRNA and a retrovirus expressing BCL-W (+) or empty vector control (–). Following the addition of Dox (+) or DMSO vehicle control (–), Western blotting for the indicated proteins (E) and MTS assays (F, in quadruplicate) were performed. Data shown are representative of 2 to 3 independent experiments. Error bars for B–D and F indicate the SD. *P < 6.4 × 10–4 (B), *P < 0.025 (C), *P < 0.034 (D), and *P < 0.0036 (F), by t test comparing shBW and Dox with shBW and vehicle.