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. 2017 Jan 17;127(2):623–634. doi: 10.1172/JCI88428

Figure 3. Matriptase cleaves EpCAM.

Figure 3

(A) Recombinant murine EpCAM-Ig was incubated with or without recombinant matriptase in “matriptase reaction buffer” at 37°C for 1 hour. Reactants were then resolved using SDS-PAGE and stained with Coomassie blue. (B) EpCAM-Ig was incubated with indicated amounts of recombinant matriptase or recombinant prostasin in “prostasin reaction buffer” at 37°C for 1 hour. Reactants were resolved via SDS-PAGE and stained with Coomassie blue. (C) Recombinant EpCAM-Ig and recombinant matriptase were coincubated as in A, and residual proteins were resolved using SDS-PAGE, transferred onto a PVDF membrane, and stained with Coomassie blue. Representative data from 1 of 3 experiments are shown in A–C. (D) The N-terminal amino acid sequence of eluted RE2 was determined, and alignment of the N-terminal sequence of RE2 with that of murine EpCAM is depicted. X indicates an indeterminate amino acid.