Figure 8. Transcriptome analysis after PO in murine hepatic tissue.

Tissue was harvested from clamped and nonclamped murine cohorts. (A) Relevant canonical pathways predicted by Ingenuity software in hepatic samples from mice after PO under insulin- (black bars) and noninsulin-stimulated (gray bars) conditions. (B) Predicted canonical pathways with a P value of less than 0.05 during insulin-stimulated (turquoise) and noninsulin-stimulated conditions (blue). (C) Noteworthy predicted upstream regulators and (D) predicted upstream regulators with a P value of less than 0.01 (asterisk denotes an activation Z score above 1.9). (E) Genes that were upregulated in both cohorts (green), downregulated in both cohorts (yellow), and downregulated under insulin-stimulated, but upregulated under noninsulin-stimulated, conditions (green and yellow, respectively) after PO treatment, with a P value of less than 0.05, a fold change greater than 1.3, and an average expression in at least 1 group of greater than 4 (n = 9–10, 273, and 327 probe sets, respectively).