Evaluation of the antiproliferative effects of 1 in cancer cells. Effect of pachycladin A treatment on the growth of (A) MDA-MB-231, (B) MDA-MB-468, (C) BT-474, (D) MCF-7, (E) SKBR3 and (F) T-47D breast cancer cells after 48 h. Effect of pachycladin A treatment on the growth of HeLa cervical cancer cells after (G) 48 and (H) 72 h. Viable cell count was determined using MTT assay. Error bars indicate the S.D. of N=3/dose. Oleocanthal and nocodazole were used as positive controls at doses selected based on earlier studies [20, 21].