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Evaluation of the pro-apoptotic effects of 1 in HeLa cells. Effect of 1 on TUNEL staining after (A) 48 h and (B) 72 h using TUNEL assay. Effect of 1 on cleaved caspase-3 levels after (C) 48 h and (D) 72 h using an immunosorbent fluorometric assay. (E) Effect of 1 on AIF release from the mitochondria after 72 h using an ELISA assay.
