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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Trends Biochem Sci. 2016 Nov 1;42(2):111–129. doi: 10.1016/j.tibs.2016.09.010

Figure 3. Design of an IFP protease indicator.

Figure 3

(A) Cartoon showing the cleavage and activation of iCasper. iCasper enables imaging of caspase-3 activation during apoptosis, one type of programed cell death. (B) iCasper enables the visualization of the spatiotemporal dynamics of apoptosis in vivo. Time-lapse images of neurons in the ventral nerve cord in Drosophila reveal caspase activation and apoptotic cell shape change during CNS development. The neuron undergoing apoptosis is indicated with the arrow. The number at the top right corner of each panel refers to time (h:min). Note that the neurons also express membrane targeted GFP, which is much brighter than the split GFP fluorescence from iCasper. Scale bar, 10 µm.