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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Trends Biochem Sci. 2016 Nov 1;42(2):111–129. doi: 10.1016/j.tibs.2016.09.010

Figure 7. Designs of FP-based GEVIs.

Figure 7

(A) In FlaSh/Flare/SPARC, GFP is inserted in an intracellular segment of a homotetrameric potassium channel (FlaSh/Flare) or of a pseudotetrameric sodium channel (SPARC). Only one repeat of the 6-helix transmembrane motif is shown for clarity. (B) In VSFP1/2, a FRET pair is fused to the intracellular segment following a 4-helix VSD. (C) VSFP-Butterfly is composed of a FRET pair of FPs (YFP and RFP) fused to the termini of the VSD. (D) In VSFP3/ArcLight, a single FP is fused following the VSD. (E) In ASAP1, a circularly permuted GFP (cpGFP) is inserted into an extracellular loop of the VSD.