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. 2017 Jan 28;8:15. doi: 10.1186/s13287-017-0484-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — FLIM of NAD(P)H in MSCs during chondrogenic differentiation. a Pseudocolor-coded images of the free (t1) and protein-bound (t2) forms of NAD(P)H. Field of view 213*213 μm (512*512 pixels). b Dynamics of the fluorescence lifetimes of free and protein-bound forms of NAD(P)H and fluorescence lifetime contributions of the protein-bound forms of NAD(P)H in MSCs. Mean ± SD. ^*Statistically significant difference with undifferentiated MSCs on the same day. P values are shown. MSCs mesenchymal stem cells, NAD(P)H reduced nicotinamide adenine dinucleotide (phosphate)