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. 2017 Jan 28;8:17. doi: 10.1186/s13287-017-0476-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The culture and characterization of cardiosphere-derived cells. a Cells grew from the explant on top of a layer of stromal-like cells after 7 days. Cardiospheres formed after 4 days in suspension culture on poly-d-lysine-coated dishes. Cardiosphere-derived cells become confluent after 5 to 7 days. Scale bar = 100 μm. b Flow cytometric analysis showed that cardiosphere-derived cells were positive for CD29, CD105, and CD90, and negative for CD31, CD45, and CD34. GFP green fluorescent protein