Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 28;8:17. doi: 10.1186/s13287-017-0476-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 6 — Metformin (MET) treatment exerted an anti-apoptotic effect on CDCs subjected to oxidative stress by activating AMPK. a1 Annexin V-PI double staining assay of CDCs treated with H₂O₂, analyzed by flow cytometric analysis. a2 Average percentages of apoptotic CDCs. n = 9. b Representative images (b1) and quantification (b2) of TUNEL staining in CDCs subjected to H₂O₂ treatment. Cell nuclei were stained with DAPI (blue) and TUNEL⁺ nuclei were labeled with TMR (green). TUNEL positive rate = (TUNEL-positive nuclei/DAPI-positive nuclei) × 100%. n = 12. Scale bar = 30 μm. c Cell viability of CDCs detected with CCK8 analysis. The data were normalized to 100% of the control group. Data were analyzed by one-way ANOVA with post-hoc comparisons by the Tukey’s test. *P < 0.05 vs. control; ^# P < 0.05 vs. H₂O₂. CC compound C