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. 2017 Jan 28;8:2. doi: 10.1186/s13227-017-0065-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Expression profiling of branching-related genes at unbranched stage in O. barthii and O. glaberrima. qRT-PCR analysis of OSH1, LAX1, SPL14, APO2, TAW1, OsMADS22, OsMADS55, miR529 and miR156 accumulation levels at stage 1 (unbranched stage with elongation of rachis meristem and formation of primary branch meristems) in O. barthii (gray lines) and in O. glaberrima (orange lines). Target mRNA and small RNA accumulation levels were normalized using rice Actin gene (LOC_Os03g50885) transcript and mature miR159 microRNA accumulation levels, respectively. For mRNAs, the graph is divided into two sections due to scale range. Statistical significances (i.e., t test) between the two species for the relative expression levels of each gene are as follows: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001