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. Author manuscript; available in PMC: 2017 Jan 28.
Published in final edited form as: J Am Chem Soc. 2016 Dec 5;138(49):15841–15844. doi: 10.1021/jacs.6b10589

Figure 2.

Figure 2

hCES1 suppresses the proteome-wide reactivity of probe 4. Ramos cells co-cultured with HEK293T cells expressing hCES1, Met-AP2, or mock-transfected (−) were treated with 3 or 4 (1 μM, 0–24 h). Gel-based ABPP revealed rapid BTK engagement and time-dependent increases in proteome-wide reactivity for both probes, except for 4 in the presence of CES1, where proteome-wide reactivity was blocked.