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. Author manuscript; available in PMC: 2017 Jan 28.
Published in final edited form as: J Am Chem Soc. 2016 Dec 5;138(49):15841–15844. doi: 10.1021/jacs.6b10589

Figure 3.

Figure 3

Characterization and hCES1-dependency of targets of 3 and 4. A, Representative MS1 spectra for probe targets. Ratios > 20 are assigned as 20 values. B, The hCES1-sensitivity of targets of 3 and 4. Proteins showing ratios of ≥ 2.5 in MetAP2/hCES1 ABPP-SILAC experiments were assigned as CES-sensitive. C. Comparison of the reactivity of 3 and 4. Co-cultures of Ramos and HEK293T cells expressing hCES1 or MetAP2 were treated with 3 or 4 (1 μM, 24 h). Average SILAC ratios for proteins from three experiments are shown.