Figure 3.

Loss of Cttn and HS1 does not affect platelet function. The loss of Cttn alone or both Cttn and HS1 had no effect on the aggregation (A & B) or α-granule secretion (C) of platelets to G-protein coupled or tyrosine kinase linked receptor agonists. Aggregation data are expressed as % final aggregation 6 mins after agonist addition. Alpha granule secretion data are % of WT values measured by flow cytometry 2 mins after addition of agonist. (D) The loss of Cttn and HS1 had no effect on pro-coagulant surface generation as phosphatidylserine exposure was not affected by loss of both cortactin and HS1. (E) Clot retraction in PRP following stimulation by thrombin was unaffected by loss of Cttn or Cttn and HS1. All data are presented as mean ± SEM, n = 3.