Table 4.
Human liver chloride concentration and EC50 values of various anions in protecting human cytosolic GSTZ1 with EGT/EGT haplotype from DCA-induced inactivation.
| Anion | EC50 (mM) (n=3) | Hepatic concentration (mM) |
|
|---|---|---|---|
| Cytosol (n=97) | Mitochondria (n=97) | ||
| Chloride | 15.0 ± 3.1A | 105.2 ± 62.4 | 4.2 ± 3.8 |
| Bromide | 1.3 ± 0.3B | - | - |
| Iodide | 0.14 ± 0.06B | - | - |
| Sulfite | 9.6 ± 1.1C | - | - |
Values shown are mean ± S.D.; EC50 is the concentration of anion that protected half the cytosolic GSTZ1 from inactivation following incubation for 2 hours with 0.5 mM DCA. Different superscript letters indicate significant differences in EC50 between anions, p<0.01, calculated by one-way ANOVA analysis of paired samples with Bonferroni's multiple comparison test. EC50 data is from Zhong et al. (2014a) and chloride concentration data from Jahn et al. (2015).