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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Arthritis Rheumatol. 2017 Feb;69(2):352–361. doi: 10.1002/art.39844

Figure 1.

Figure 1

MIF levels in conditioned media from normal and OA chondrocyte and meniscal cell cultures and in normal and OA cartilage sections. A. Serum-free medium was collected after 16 hours of culture from unstimulated confluent monolayers of normal and OA chondrocytes and meniscal cells. MIF protein in media was measured by human MIF ELISA. Horizontal lines on the graph represent average MIF level for each tissue type; individual data points are shown. Normal chondrocytes: n=12 donors; OA chondrocytes: n=9 donors; normal meniscus: n=8 donors; OA meniscus: n=9 donors. Statistical significance was evaluated by unpaired t-test within tissue types. B. Serum-free conditioned medium was collected after 48 hours of culture from unstimulated human and OA cartilage explants. MIF protein in media was measured by human MIF ELISA and was normalized to the DNA content of the explants. Individual data points are shown. Normal cartilage explants: n=16 donors; OA cartilage explants: n=7 donors. Statistical significance was evaluated by unpaired t-test, and no outliers were identified by the Grubbs outlier test. Immunohistochemistry results for MIF in sections of (C) human normal knee cartilage and (D) human OA knee cartilage.