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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Arthritis Rheumatol. 2017 Feb;69(2):352–361. doi: 10.1002/art.39844

Figure 2.

Figure 2

Mice with Mif deletion are protected from age-related OA. A. Representative histologic images of knee joint sections in 12-month old Mif−/− and WT mice. (i) WT mouse, medial tibial plateau (MTP) articular cartilage structure (ACS) score 11. Degradation and loss of articular cartilage (arrow), osteophyte formation (arrowhead), and thickening of subchondral bone (bracket). (H&E) (ii) Mif−/− mouse, ACS score 3. Normal subchondral bone thickness (bracket). (H&E) (iii) WT mouse section immunostained for MIF. Intracellular MIF is present in meniscus (arrow) and articular cartilage (arrowhead). (iv) Mif−/− mouse. Lack of MIF immunopositivity in meniscus (arrow) and cartilage (arrowhead). B. Articular cartilage structure scores in Mif−/− and WT mice. The medial tibial plateau of mid-coronal stifle sections was scored using the articular cartilage structure (ACS) score. 12-month old WT: n=13; 12-month old Mif−/−: n=13; 22-month old WT: n=14; 22-month old Mif−/−: n=16. C. Osteophyte scores in Mif−/− and WT mice from (B). The summed scores from the medial tibial plateau (MTP) and medial femoral condyle (MFC) are presented. Horizontal lines on graph represent the mean of each group. Significant differences were determined by Kruskal-Wallis nonparametric one-way ANOVA followed by Dunn’s multiple comparisons test.