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. Author manuscript; available in PMC: 2018 Mar 30.
Published in final edited form as: Behav Brain Res. 2016 Jul 28;322(Pt B):206–211. doi: 10.1016/j.bbr.2016.07.042

Figure 1.

Figure 1

Constitutively-active CREB expression reduces peak and slow AHP evoked with 15 suprathreshold current injections at 50Hz in CA1 pyramidal neurons from young adult rats. Whole-cell current clamp recording experiments were conducted 2 weeks after the injection of a lentiviral vector to express VP16-CREB (caCREB) in CA1 of the hippocampus. The caCREB sequence was downstream from a hSyn promoter, followed by an EGFP reporter (downstream of its own hSyn promoter), to allow for visualization of infected cells. a) Example stitched 10x confocal image of EGFP expression 2 weeks post-injection of lentivirus into dorsal CA1 area. Summed z-stack of infected CA1 pyramidal neurons (inset). Left scale bar 250 μm, right scale bar 100 μm. b) Peak AHP amplitudes measured from caCREB-infected cells (green: n = 11) are smaller than that of neighboring uninfected control cells (blue: n = 10) (unpaired t-test: * p < 0.05). c) Slow AHP amplitudes measured from caCREB-infected cells are also smaller than that of neighboring uninfected control cells (** p < 0.01). d) Representative traces from AHP recordings made from caCREB-infected and control cells, arrow indicates 1 s time point where slow AHP was measured. Bars in b and c represent mean ± S.E.M.