Skip to main content
. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Microbes Infect. 2016 Dec 21;19(2):101–109. doi: 10.1016/j.micinf.2016.12.002

Fig. 1.

Fig. 1

Production of Coa, vWbp and ClfA by S. epidermidis and S. simulans. (A) Diagram illustrating plasmid inserts carrying the coa, vwb or clfA genes from S. aureus Newman. (B) Culture supernatants were examined by immunoblotting with antibodies specific for Coa (α-Coa) and vWbp (α-vWbp) while whole culture lysates were used to examine ClfA production using antibodies specific for ClfA (α-ClfA). Cells cultures were normalized A600 4 and S. aureus samples were diluted 10 times for Coa and vWbp or concentrated 5 times for ClfA analyses as compared to S. epidermidis and S. simulans extracts. Numbers to the left of blots indicate the position of molecular markers in kDa.