Table 5.

Summary table of key responses affected by process parameter interactions as well as the observed single parameter effects and literature comparison

	Observed single parameter effects	Process parameter interactions	Literature
µaverage	Higher at increased pH, and pO2; quadratic pCO2 effects	pH × pCO2	Similar single effects [13, 15, 18, 25]
q p	Higher at increased pH, and pO2; quadratic pCO2 effects	pH × pCO2	Similar single effects [15, 20]; Contrary single effects [13, 25]
q AA	Higher uptake rates and lower production rates of several amino acids at increased pH and pO2; contrary effects of pCO2	pH × pCO2/pH × pO2	Similar single effects of process pH [13]
mAb acidic charge variant (ACV 2)	Higher at increased process pH	pH × pCO2	No literature available since variant identity is unknown
mAb sialylation (bG1FSA_2)	Quadratic pH effects	pH × pCO2	Similar interaction effect [19]

Key responses with process parameter interaction effects: average specific cell growth (µ _average), specific productivity (q _p), specific amino acid consumption/production rates (q _AA), acidic charge variant 2 and mAb sialylation variant bG1FSA_2. Effects are only valid inside of the experimental design space. Contradictions to/in literature may derive from cell line specific effects. More detailed information is presented in the corresponding paragraphs of the “Results and discussion” section