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Comparison of direct and sandwich IgG subclass assays.
| IgG1 beads | IgG2 beads | IgG3 beads | IgG4 beads | IgM beads | ||
|---|---|---|---|---|---|---|
| Sandwich assay | IgG1 HP6001 |
100% | 14% | 41% | 15% | 2% |
| IgG2 HP6002 |
8% | 100% | 27% | 25% | 2% | |
| IgG3 HP6047 |
1% | 0% | 100% | 4% | 0% | |
| IgG4 HP6023 |
1% | 1% | 5% | 100% | 0% | |
|
| ||||||
| Direct assay | IgG1 4E3 |
100% | 228% | 260% | 5% | 1% |
| IgG1 HP6001 |
100% | 2% | 88% | 3% | 1% | |
| IgG2 HP6002 |
5% | 100% | 105% | 126% | 6% | |
| IgG2 31-7-4 |
14% | 100% | 245% | 185% | 2% | |
| IgG3 HP6050 |
0% | 0% | 100% | 1% | 0% | |
| IgG4 HP6023 |
0% | 0% | 2% | 100% | 0% | |
