Table 1.
qRT-PCR Primers used for gene amplification of commonly up- and down-regulated genes across treatment and time in nfe2 knockout. Primer sequences are written 5′–3′ and amplicon size is shown.
| Gene | Primer Sequence (5′ – 3′) | Amplicon Size |
|---|---|---|
| b2m | F: CTGAAGAACGGACAGGTTATGT | 125 |
| R: ACGCTGCAGGTATATTCATCTC | ||
| cfap70 | F:CACGGAGACCTTACTGACATAGAG | 187 |
| R: GTACCGTCTGAGTGACTTTCATG | ||
| cyp4v7 | F: AGCAATTCCAGACACCTTGAC | 135 |
| R: TGGAGAAATGGAAGGTTGGAG | ||
| dhx40 | F: CACGCAGCTACCTCAGTACTTG | 85 |
| R: GACACTCTCTGGGCTACAGTG | ||
| dnajb9b | F: CTTTCACAAACTAGCCATGCG | 138 |
| R: GCTTCTCGTCTGATCGTATTCC | ||
| fhad1 | F: CTACCTCTGTTTCACCCCATC | 129 |
| R: CATCCTCTGTTAGCACTCCAG | ||
| gpr19 | F: TCTACGTGCTGCTGTCCATC | 243 |
| R: AGGAACCCAAACAGCAAATG | ||
| nlrp12 | F: CTTCAGGCCTGTTTCTCCAG | 197 |
| R: AGGAACAGTGTGGGGAGATG | ||
| slc16a6a | F: TCAACTGCATTCACCAGCTC | 184 |
| R: GGAGCGAAAGCAAATGTAGC | ||
| slc35f4 | F: TTTATGGGTGTGGCCTTAGC | 214 |
| R: CCCAGGGTAAAGAGGAGAGG | ||
| spi-c | F: TGGCTCTGTGGACACTTCTG | 202 |
| R: TACTGCTGGGCAGTGACTTG |