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Enzyme kinetics of pure recombinant BYK (rBYK)
| Source of BYK | Enzyme activity (mM/ml/min) | KM (mM) | Vmax (mM/mg/min) |
|---|---|---|---|
| rBYK | 0.020±0.0100 | 0.750±0.10 | 0.42±0.10 |
| rBYK+membrane fraction | 0.064±0.009 | 0.206±0.07 | 1.006±0.43 |
| rBYK+membrane fraction+ substrate BYKs | 0.703±0.080 | 0.333±0.08 | 9.53±1.45 |
Values are the mean±SD from three determinations. 1, basal rBYK kinetics; 2, auto-phosphorylation kinetics; 3, substrate-level phosphorylation kinetics.
