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. Author manuscript; available in PMC: 2017 Jan 29.
Published in final edited form as: Nat Protoc. 2012 Jul 19;7(8):1502–1510. doi: 10.1038/nprot.2012.084

Figure 1. Strain PF405 Genetics.

Figure 1

The qC1-balanced region of chromosome is shown in green. We used a version of qC1 with the qIs26 insertion that has the lag-2::GFP transgene and the dominant rol-6 allele su1006. The qIs26 insertion site created a recessive lethal allele such that qC1[qIs26] homozygotes are inviable. Heterozygotes have the Rol-6 phenotype and express GFP. Animals that have lost the balancer do not express GFP, are uncoordinated, and are mel-28 (and thus produce only dead embryos).