(a, c, e) Heterozygous mel-28 unc-32/qC1[qIs26] adults (Rol-6 phenotype). (b, d, f) Homozygous mel-28 unc-32 adults from the PF405 strain. Images were captured in brightfield (a, b, d, f), and with GFP filters (c–d). (a–d) Animals were anesthetized with levamisole and immobilized on an agar pad. (e–f) characteristic behavior of Rol-6 (e) and Unc (f) adults on an NGM plate .