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. Author manuscript; available in PMC: 2017 Jan 29.
Published in final edited form as: Nat Protoc. 2012 Jul 19;7(8):1502–1510. doi: 10.1038/nprot.2012.084

Figure 3. Preparation of C. elegans for laFACS.

Figure 3

(a) After a ~4 minute treatment with sodium hypochlorite and agitation, most adults have burst and freed their embryos (arrowhead). (b) After overnight incubation in M9, many L1 larvae have hatched but large segments of adult corpses (asterisk) remain. (c) After filtering, only small debris and L1 larvae remain. Scale bar = 200 µm (a).100 µm (b–c).