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NMR titration data recorded for the (a) D11, (b) Y14, (c) E18, (d) S72, and (e) D171 residues from HisJ in the presence of excess l‐His, l‐Arg, l‐Lys, and l‐Orn, respectively. For each representative data apo‐HisJ appears in black, whereas 0.5, 1.0, 2.0, and 16 times excess ligands are shown in cyan, red, green and blue, respectively. The arrow indicates the movement (fast exchange) of apo‐HisJ peaks for the specific residues upon successive ligand addition.
