Figure 3.

The known macro domain structures allow for structure solution of SPX^ScVtc 4 by molecular replacement. (A) Structural superposition of different, previously described macro H2A1.1 macro domain structures indicates that N‐ and C‐termini are flexible. R.m.s.d. is between 0.7‐2.6 Å comparing 180 corresponding C_α atoms. (B) Table summary of molecular replacement calculations using the different known macroH2A1.1 structures. 1xRFZ: rotation function Z‐score for the 1^st molecule to be placed. 1xTFZ: translation function Z‐score for the 1^st molecule to be placed. 2xRFZ: rotation function Z‐score for the 2^nd molecule to be placed. 2xTFZ: translation function Z‐score for the 2^nd molecule. LLG: Log Likelihood Gain of the refined solution. (C) C_α traces of two SPX^ScVtc4‐macro molecules (SPX domain are shown in light‐blue and yellow, the C‐terminal macro domains in dark‐blue and orange, respectively) related by a pseudo 2‐fold axis in the asymmetric unit of the I2₁2₁2₁ crystal form. The central core helices of the SPX domains are highlighted (ribbon diagrams).