Figure 8.

Michaelis–Menten plot of chymotrypsin in the presence of Hg²⁺, in 50 mM MOPS, pH 7.14. Substrate was the amide succ‐phe, data were fit to Eq. (2). Black circles = control (without Hg²⁺), blue squares = 0.1 mM Hg²⁺, green diamonds = 0.2 mM Hg²⁺, black Xs = 0.3 mM Hg²⁺. Final [chymotrypsin] remaining in solution, and all fitted Michaelis–Menten parameters are listed in Table II. For the 0.3 mM Hg²⁺ curve, V _max and K _m returned P values ≫ 1, indicating that these points do not fit hyperbolic saturation.