Figure 4.

Biochemical reconstitution of the ctUTP‐A and ctUTP‐B complex. Thermophilic proteins were recombinantly co‐expressed in E. coli or S. cerevisiae and co‐purified (for details, see “Materials and Methods”). The dimeric and trimeric reconstituted complexes of ctUTP‐A (A) and ctUTP‐B (B) were analyzed by SDS‐PAGE and subsequent Coomassie blue staining. The label at the right indicates the position of the protein with its tag. A protein A tagged protein, which gets cleaved from its tag during purification is marked with an asterisk. (C) For the reconstitution of the complete ctUTP‐A (left panel) and ctUTP‐B (right panel) complex, all members were recombinantly co‐expressed in yeast and isolated using a split tag approach.