Figure 2.

SDS‐polyacrylamide gel electrophoresis of neck domain co‐purification with the extracellular portion of DC‐SIGN on mannose‐Sepharose. The extracellular fragment of DC‐SIGN alone or the extracellular fragment and the neck domain fragment mixed in a ratio of ∼4:1 by weight were dissolved in 8 M Urea containing 50 mM Tris, pH 6.8, and dialyzed extensively against Ca²⁺‐containing loading buffer. The renatured samples were fractionated on a 1‐mL column of mannose Sepharose that was washed with four 1‐mL aliquots of Ca²⁺‐containing loading buffer and eluted with four 0.5‐mL aliquots of EDTA‐containing elution buffer. Aliquots were run on a 17.5% SDS‐polyacrylamide gel, which was stained with Coomassie blue.