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. 2016 Aug 26;173(19):2859–2879. doi: 10.1111/bph.13562

Figure 4.

Figure 4

ASCs treatment improves pulmonary vascular remodelling in PH in a paracrine fashion. ASCs or CM were injected via a jugular cannula, 2 weeks following the MCT insult. Following 4 weeks of MCT‐insult, pulmonary vessel wall thickness was assessed by IHC and IF staining. (A) Pulmonary vessel wall thickness. (B) Pericyte coverage; NG2 (green, pericyte marker), αSMA (red, smooth muscle marker). Immunofluorescence images were viewed under a spinning disc confocal microscope. (B.a), (B.e), (B.i), (B.m) and (B.q) represent the DAPI staining. (B.b), (B.f), (B.j), (B.n) and (B.r) represent the αSMA with DAPI staining. (B.c), (B.g), (B.k), (B.o) and (B.s) represent the NG2 with DAPI staining. (B.d), (B.h), (B.l), (B.p) and (B.t) represent the merged image of αSMA and NG2 with DAPI. Inserts in (h) and (l) represent the contact point between αSMA and NG2 stained cells. (C) and (D) represent the pulmonary vessel wall thickness and pericyte coverage in all the experimental groups. Data presented in (C) and (D) are mean ± SEM, (10 randomly chosen fields from each animal and n = 5 animals). Scale bar is 25 μm. * P value of ≤ 0.05, comparing MCT and MCT + M versus control. # P value of ≤ 0.05, comparing MCT + A, and MCT + CM versus MCT. @ P value ≤ 0.05, comparing MCT + CM versus MCT + M.