Figure 10.

Characterization of ASCs‐CM by cytokine array. In order to profile the contents of CM, we used the cytokine array system. CM, conditioned from the serum‐starved ASCs were analysed for the protein contents. A total of 200 μg of protein in 285 μL were loaded in the cytokine array, and the instructions were followed as described in the kit. 285 μL of concentrated plain culture media (without serum) was used for the control array. (A) Presence of different cytokines and growth factors in control media and in CM. (B) Mean pixel density of the respective cytokines and growth factors present in CM as compared with the control media. Mean pixel density is the average of duplicates. (C) X and Y axis coordinates of cytokine array strip.