Figure 3. Sensitivity analysis of the isolated mitochondrial model .

The simulation conditions were the same as those of Fig. 2 A, with [Ca²⁺]_cyt = 1.0 μm. Sensitivity (S) for parameter X (X = $\mathrm{m}\dot{V}{}_{{\mathrm{O}}_2}$, NADH and ΔΨ) was calculated as the relative change by ±5% change of the expression level of each component: $S=\frac{|X_{+5\%}-X_{-5\%}|}{0.1X_{\mathrm{original}}}$.