Skip to main content
. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Virology. 2016 Dec 30;502:133–143. doi: 10.1016/j.virol.2016.12.022

Figure 2. NF-κB localizes to perinuclear compartments in cardiac myocytes.

Figure 2

Figure 2

(A) Primary cardiac myocyte, cardiac fibroblast, or skeletal muscle cultures were fixed for immunofluorescent microscopy using antibodies against p65 and vimentin. Nuclei were counterstained with DAPI. Scale bar = 20 μm. (B) Primary cardiac myocyte cultures generated from the indicated mouse strain were immunostained as in panel A and the percentage of cardiac myocytes (defined as ‘vimentin negative’) were scored according to the localization of p65 in each cardiac myocyte (n = 104 – 214 per case) for a representative experiment. (C) Cardiac myocyte cultures were generated from either ‘fetal and neonatal’ or ‘one- and two-day-old’ wild-type mice and scored as in panel B.