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. Author manuscript; available in PMC: 2018 Feb 1.

Published in final edited form as: Virology. 2016 Dec 30;502:133–143. doi: 10.1016/j.virol.2016.12.022

Primary cardiac cultures were infected with reovirus T3D or media alone (‘mock’). After incubation for 2 h, the inoculum was removed and replaced with either media alone or media containing 20 nM LMB. Cells were incubated for another 6 h prior to fixation and immunostained as indicated. The apparent co-localization of RelA within reovirus viral factories is due to cross-reactivity of the p65 antibody with a reovirus protein (data not shown). Scale bar = 10 μm. The percentage of cardiac fibroblasts (n = 8 – 104 cells per condition) or cardiac myocytes (n = 18 – 48 cells per condition) displaying nuclear p65 is indicated for a representative of two independent experiments. For cases that received reovirus inoculum, only cells positive for reovirus antigen were scored.

Primary cardiac cultures were infected with reovirus T3D or media alone (‘mock’). After incubation for 2 h, the inoculum was removed and replaced with either media alone or media containing 20 nM LMB. Cells were incubated for another 6 h prior to fixation and immunostained as indicated. The apparent co-localization of RelA within reovirus viral factories is due to cross-reactivity of the p65 antibody with a reovirus protein (data not shown). Scale bar = 10 μm. The percentage of cardiac fibroblasts (n = 8 – 104 cells per condition) or cardiac myocytes (n = 18 – 48 cells per condition) displaying nuclear p65 is indicated for a representative of two independent experiments. For cases that received reovirus inoculum, only cells positive for reovirus antigen were scored.