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. 2017 Jan 30;5:2. doi: 10.3389/fcell.2017.00002

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Copyright © 2017 Valarmathi, Fuseler, Davis and Price.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron microscopic (TEM) analysis of prevascularized CCCs. TEM analysis of day 14 vasculogenic hCMVECs/hMSCs CCC cultures revealed an elongated vessel-like structure composed of endothelial cells, which were arranged on either side of a linear slit-like space (black arrows, A). Randomly oriented bundles of collagen fibrils were evident on either side of this elongated capillary. Note the most characteristic feature of endothelial cell, small membrane-bound vesicles, resembled a little flask, or caveola, and occupied up to a quarter of the endothelial cells (B, insert). The interdigitating endothelial cells showed the junctional complex (C, lower magnification). The typical adherent junction could be visualized between two overlapping endothelial cell processes (D, higher magnification, insert). (A, scale bar 2 μm; B–D, scale bar 500 nm).