Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 29;108(1):12–22. doi: 10.1111/cas.13117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Effects of NS5ABP37 on cell proliferation, apoptosis, and cycle distribution in hepatocellular carcinoma cell lines. HepG2 and L02 cells were transiently transfected with pENTER (normal control [NC]), pENTER‐NS5ABP37, siRNA control (siNC), or siRNA‐NS5ABP37 for 48 h. (A) Cell proliferation was measured using a CCK‐8 kit. Cell apoptosis was assessed with an annexin V‐FITC/7‐AAD kit by flow cytometry (B1), and quantitated (B2). Caspase‐3 activity was detected by caspase‐3/7 activity assay (C) and Western blot using anti‐cleaved caspase‐3 antibody (D). (E1,E2) Cell cycle distribution was measured by flow cytometry and analyzed with ModFit software. (F) Cell cycle‐related proteins, including cyclin D1, cyclin A, cyclin E, and CDK2 were detected by Western blotting. Data are mean ± SD from triplicate experiments. *P < 0.05, **P < 0.005, ***P < 0.0001.