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. 2017 Jan 29;108(1):151–155. doi: 10.1111/cas.13103

Figure 1.

Figure 1

Effects of double decoy receptor ALK1FLT1‐Fc on vascular endothelial growth factor (VEGF) and bone morphogenetic protein‐9 (BMP‐9) signals in endothelial cells. (a) Schematic illustrations of constructed Fc chimeric proteins. (b–e) The conditioned media of HEK293T cells transiently transfected with expression vectors encoding control‐Fc (Ctrl‐Fc), Fms‐related tyrosine kinase 1 (FLT1)‐Fc, activin receptor‐like kinase 1 (ALK1)‐Fc, or ALK1FLT1‐Fc were collected, followed by determination of Fc protein concentrations by ELISA. HUVECs cultured with Fc fusion proteins were treated with BMP‐9 for 4 h or VEGF for 1 h, followed by quantitative RT‐PCR analyses for EGR3 (b, d) and ID1 (c, e), respectively. Error bars indicate SD. *P < 0.05. n.s., not significant.