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Assessment of viral replication in mesothelioma cells. NCI‐H226 cells (3 × 105) were infected with replication‐competent Ads (Ad5VEGFE1 [○] or Ad5/3VEGFE1 [∆]) or non‐replicative Ad (Ad5/3CMVLuc [■]) at an MOI of 10 for 3 h. Then, cells were divided into three portions and re‐plated in a 24‐well plate. Cells were harvested, and viral replication was assessed every 3 days in a standard plaque assay.
