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. 2017 Jan 30;8:6. doi: 10.3389/fgene.2017.00006

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Copyright © 2017 Checcucci, Azzarello, Bazzicalupo, De Carlo, Emiliani, Mancuso, Spini, Viti and Mengoni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogeny of the ACC deaminase gene cassette. (A) Maximum Likelihood phylogenetic reconstruction of acdS gene sequences The phylogenetic analysis is based upon acdS orthologs found in Rhizobiaceae. The dendreogram hightlight the presence of two clades of the gene in S. meliloti., vertical lines (B) Genomic context of the acdS orthologs from the two clades of acdS orthologs in S. meliloti strains and from Bradirhizobium and Rhizobium leguminosarum strains. acdS region map pointed out the presence of MGE (Mobile Genetic Elements) close to acdS. The length (bp) of genes and intergenic regions is indicated, as well as ORF orientation (using arrows). The GTR+G model has been chosen for the reconstructions after model test evaluation (Supplementary Data 1).